Research Interests

• Molecular cloning, heterologous expression and production of biocatalysts that are implied in the degradation and/or modification of lignocellulosic biomass fractions for the production of bioactive compounds

• Biochemical characterization of enzymes (biochemical assay development, synergistic and inhibition kinetics, hydrolysis of natural substrates)

• Production and characterization of novel biocatalysts with oxidative activity and investigation of their applications in nanocellulose production

• Development of fermentation processes for the production of value-added products from the biomass sugar streams (lactic acid, omega-3 fatty acids)

• Evaluation of enzymatic activities on biomass fractions and development of customized cocktails towards the production of prebiotic compounds

Education

• 06/2010 – 01/2015

Ph.D., Biotechnology, School of Chemical Engineering, National Technical University of Athens (NTUA), Athens, Greece

Thesis title: “Construction of microbial platform for the production of thermophilic enzymes implicated in lignocellulose degradation”,

Supervisor: Professor P. Christakopoulos

• 09/2008 – 07/2010

MSc., Microbial Biotechnology, National Kapodistrian University of Athens (UoA), Athens, Greece Thesis title (NTUA, School of Chemical Engineering, Biotechnology Laboratory): “Heterologous overexpression and catalytic characterization of a thermophilic GH7 endoglucanase from Sporotrichum thermophile”

Advisor: Professor P. Christakopoulos

• 09/2002 – 04/2008

BSc., Biology, National Kapodistrian University of Athens (UoA), Athens, Greece

Diploma thesis title (UoA, Faculty of Biology, Bioinformatics Laboratory): “Gene polymorphisms and correlated diseases”

Advisor : Prof. S. Hamodrakas

Awards

• 2017 – 2019: Scholarship for 2-year postdoctoral research from the State Scholarship’s Foundation, Greece.

• 02/2016 – 01/2017: Grant for 1-year postdoctoral research from Kempe Foundations (SMK-537).

• 02/2016 – 04/2016: Grant for a 3-month Short Term Scientific Mission in Tor Vergata University, Rome, Italy by COST Action FP1306.

• 02/2015 – 01/2016: Grant for 1-year postdoctoral research from Swedish Research Council Formas (2014-148).

• 02/2014 – 09/2014: Scholarship for a 6-month Short Term Scientific Mission to Lulea University of Technology by the State Scholarship’s Foundation, Greece.

• 2014: Thomaidio Award, best scientific publication of 2014, National Technical University of Athens, Greece.

• 2010-2014: Scholarship for 3.5 years postgraduate studies by the State Scholarship’s Foundation, Greece.

Participation in Research Projects

• 2019- running: Researcher, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens (in collaboration with Laboratory of Physical Chemistry and Applied Electrochemistry) (participated in proposal design, submission and research activities): “Electrochemical Analysis for the action of Lytic Polysaccharide Monooxygenases, Echem-4-LPMO”, (3090), funded by Hellenic Foundation for Research & Innovation.

• 2018- running: Associated Principal Investigator, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens (in collaboration with Centre for Research & Technology Hellas, CERTH) (participated in proposal design and submission and performed research): “Novel Conversion Technologies of Waste Biomass to Food additives and Fine Chemicals” (1085), funded by Hellenic Foundation for Research & Innovation.

• 2018- running: Postdoctoral Research Associate, National Technical University of Athens (performed research work): “Enzymatic superficial modification of natural and synthetic polymers and their spectroscopic analysis” IKYDA (IKY-DAAD), funded by German Academic Exchange Service (DAAD).

• 2016-2017: Postdoctoral Research Associate, Lulea University of Technology (participated in proposal design and submission and performed research): “Second Generation Biofuel production From Marine Filter Feeders”, funded by Swedish Energy Agency, Sweden.

• 2016: Postdoctoral Research Associate, Lulea University of Technology (participated in proposal design and submission and performed research): “Bark biorefinery through physicochemical pretreatment and fractionation for the valorization of ‘oleochemicals’ and the additional ‘active’ ingredients” (SMK-1537), funded by Kempe Foundations, Sweden.

• February-May 2016: Research Assistant, University of Rome ‘Tor Vergata’, (performed part of the research work): “Chemoenzymatic Fractionation and Characterization of Birch Bark Lignins”.

• 2015-2016: Postdoctoral Research Associate, Lulea University of Technology (performed part of the research work): “Bioconversion of Recalcitrant Polysaccharides by Novel Oxidative Biocatalysts for the production of ethanol and other novel products”, funded by Swedish Research Council Formas, Sweden.

• 2015: Postdoctoral Research Associate, Lulea University of Technology (participated in proposal design and submission and performed research): “Efficient bioconversion of biomass insoluble polyesters with a potential use in lignocellulosic feedstock biorefineries” (2014-148), funded by Swedish Research Council Formas, Sweden.

• 2013: Research Assistant, National Technical University of Athens (performed part of the research work): “Metagenomics of ligninolytic microorganisms – Bioconversion of plant by-products into high-added value products”, THALIS PROJECT, funded by Hellenic Ministry of Education.

Employment record

• 2017 – running: Postdoctoral Researcher, School of Chemical Engineering, National Technical University of Athens, Greece

• Valorization of lignocellulosic biomass-derived sugars towards value-added products • Development of fermentation processes for the production of lactic acid and omega-3 fatty acids •Production and characterization of oligosaccharides with prebiotic activity • Production and characterization of lytic polysaccharide monooxygenases with novel enzymatic activities

• 2015 – 2017: Postdoctoral Researcher, Lulea University of Technology (LTU), Sweden

Pretreatment (hydrothermal, steam explosion, organosolv) of biomass from forest and marine environment • Isolation of biomass compounds with industrial interest (phenols, tannins, terpenes) • Enzymatic modification/depolymerization of biomass fractions for the production of bioactive compounds • Evaluation of novel enzymatic activities on biomass fractions and development of customized cocktails • Characterization of residual solid and liquid fraction after pretreatment and enzymatic transformation • Isolation and characterization of phenol and polyester biomass structures

• 02/2016 – 05/2016: Research assistant, University Tor Vergata (UNITOV), Italy

Development of analytical methods for biomass fractions characterization and evaluation of enzymatic activity (FTIR, 1H, 13C, 31P NMR 1D/2D NMR, GC-MS, GPC)

• 02/2014 – 12/2014: Research assistant, Lulea University of Technology (LTU), Sweden

Production and recovery of recombinant lignocellulose degrading enzymes (celluloses/hemicelluloses) expressed in P. pastoris yeast in bench-top fermentors • Development of tailor-made enzyme mixtures targeted towards agricultural and forest residues

• 2010 – 2014: Research assistant, Biotechnology Laboratory, School of Chemical Engineering, NTUA, Greece

Molecular cloning of genes encoding 5 cellulolytic enzymes and heterologous expression in P. pastoris • Complete characterization of produced enzymes (biochemical assay development, synergistic and inhibition kinetics, hydrolysis of natural substrates)

Teaching Experience

2020- today: Teaching assistant (under contract according to P.D. 407/80), Department of Computer Science and Biomedical Informatics, University of Thessaly (UTH), Greece:

Biology

2015- 2017: Teaching assistant, Lulea University of Technology (LTU), Sweden:

Organic Chemistry

Chemical Principles

Bioprocess Engineering

Supervision of diploma thesis for 2 undergraduate students

2013-2016: Teaching assistant, School of Chemical Engineering, National and Technical University of Athens (NTUA), Greece:

Enzyme and Microbial Technology

Basic Principles of Biotechnology

Supervision of diploma thesis for 7 undergraduate students.

Publications (Citation Index)

36. Asimakopoulou, G., Karnaouri, A., Staikos, S., Stefanidis S.D., Kalogiannis K.G., Lappas, A.A., Topakas, E. (2021) Production of omega-3 fatty acids from the microalga Crypthecodinium cohnii by utilizing both pentose and hexose sugars from agricultural residues. Fermentation, 7(4), 219

35. Karnaouri, A., Chorozian, K., Zouraris, D., Karantonis, A., Topakas, E., Rova, U., Christakopoulos, P.(2022) Lytic polysaccharide monooxygenases as powerful tools in enzymatically assisted preparation of nano-scaled cellulose from lignocellulose: A review. Bioresource Technology, 345, 126491

34. Zerva, A., Pentari, C., Ferousi, C., Nikolaivits E., Karnaouri, A., Topakas, E. (2021) Recent advances on key enzymatic activities for the utilisation of lignocellulosic biomass. Bioresour. Technol. 342, 126058

33. Dedes, G., Karnaouri, A., Marianou, A.A., Kalogiannis K.G., Michailof C.M., Lappas, A.A., Topakas, E. (2021) Conversion of organosolv pretreated hardwood biomass into 5-hydroxymethylfurfural (HMF) by combining enzymatic hydrolysis and isomerization with homogeneous catalysis. Biotechnol. Biofuels. 14(1), 172

32. Karnaouri, A., Asimakopoulou, G., Kalogiannis, K.G., Lappas, A.A., Topakas, E. (2021) Efficient production of nutraceuticals and lactic acid from lignocellulosic biomass by combining organosolv fractionation with enzymatic/fermentative routes. Bioresour. Technol. 341, 125846

31. Zouraris, D., Karnaouri, A., Pandis, P.K., Argirusis C., Topakas, E., Karantonis, A. (2021) On the interaction of lytic polysaccharide monooxygenases (LPMOs) with phosphoric acid-swollen cellulose (PASC). Journal of Electroanalytical Chemistry. 897, 115540

30. Zouraris, D., Karnaouri, A., Xydou, R., Topakas, E., Karantonis, A. Exploitation of enzymes for the production of biofuels: Electrochemical determination of kinetic parameters of LPMOs. Appl. Sci. 2021, 11(11), 4715.

29. Muraleedharan, M.N., Karnaouri, A., Piatkova, M., Ruiz-Caldas M.X., Matsakas, L., Liu, B., Rova, U., Christakopoulos, P., Mathew, A.P. (2021) Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases. Int. J. Biol. Macromol., 183, 101-109.

28. Karnaouri, A., Jalvo, B., Moritz, P., Matsakas, L., Rova, U., Hofft, O., Sourkouni, G., Maus-Friedrichs, W., Mathew, A.P., Christakopoulos, P. (2021) Lytic polysaccharide monooxygenase-assisted preparation of oxidized-cellulose nanocrystals with a high carboxyl content from the tunic of marine invertebrate Ciona intestinalis. ACS Sustainable Chem. Eng. 2020, 8(50), pp. 18400–18412.

27. Hruzova, K., Matsakas, L., Karnaouri, A., Noren F, Rova, U., Christakopoulos, P (2021) Valorization of outer tunic of the marine filter feeder Ciona intestinalis towards the production of second-generation biofuel and prebiotic oligosaccharides. Biotechnol. Biofuels 14, 32.

26. Paz, A., Karnaouri, A., Templis, C.C., Papayannakos, N., Topakas, E.Valorization of exhausted olive pomace for the production of omega-3 fatty acids by Crypthecodinium cohnii. Waste Management, 2020, 118, 435–444.

25. Hruzova, K., Matsakas, L., Karnaouri, A., Noren F, Rova, U., Christakopoulos, P (2020) Second-generation biofuel production from the marine filter feeder Ciona intestinalis. ACS Sustainable Chem. Eng. 2020, 8, 22, 8373–8380.

24. Dedes G, Karnaouri A., Topakas E, Novel routes in transformation of lignocellulosic biomass to furan platform chemicals: from pretreatment to enzyme catalysis (2020), Catalysts 2020, 10(7), 743

23. Giakoumakis G, Karnaouri A, Topakas E, Sidiras D. (2020) Simulation and optimization of combined acid pretreatment and enzymatic saccharification of medical cotton waste. Biomass Conv. Bioref. https://doi.org/10.1007/s13399-020-00694-1

22. Kalogiannis K, Karnaouri Α, Michailof C, Tzika A.M., Asimakopoulou G, Topakas E, Lappas A.A, OxiOrganosolv: A novel acid free oxidative organosolv fractionation for lignocellulose fine sugar streams (2020), Bioresour. Technol., 313, 123599

21. Karnaouri A, Asimakopoulou G,Kalogiannis K, Lappas A.A, Topakas E. (2020) Efficient D-lactic acid production by Lactobacillus delbrueckii subsp. bulgaricus through conversion of organosolv pretreated lignocellulosic biomass. Biomass and Bioenergy, 140, 105672

20. Karnaouri A, Chalima A, Kalogiannis K, Varamogianni-Mamatsi D, Lappas A, Topakas E. (2019) Utilization of organosolv pretreated lignocellulosic biomass for the production of omega-3 fatty acids by the heterotrophic marine microalga Crypthecodinium cohnii. Bioresour. Technol. 303, 122899

19. Karnaouri A, Matsakas L, Krikigianni E, Christakopoulos P, Rova U. (2020) Valorization of waste forest biomass towards the production of cello-oligosaccharides with prebiotic potential by utilizing customized enzyme cocktails. Biotechnol Biofuels 12, 285.

18. Karnaouri A, Matsakas L, Buhler S, Muraleedharan MN, Christakopoulos P, Rova U. (2019) Tailoring Celluclast® cocktail’s performance towards the production of prebiotic cello-oligosaccharides from waste forest biomass. Catalysts 9, 897.

17. Karnaouri A, Antonopoulou I, Zerva A, Dimarogona M, Topakas E, Rova U, Christakopoulos P. (2019) Thermophilic enzyme systems for efficient conversion of lignocellulose to valuable products: structural insights and future perspectives for esterases and oxidative catalysts. Bioresour. Technol. 279, 362-372.

16. Chalima A, Hatzidaki A, Karnaouri A, Topakas E. (2019) Integration of a dark fermentation effluent in a microalgal-based biorefinery for the production of high-added value omega-3 fatty acids. Applied Energy 241, 130-138.

15. Muraleedharan MN, Zouraris D, Karantonis A, Topakas E, Sandgren M, Rova U, Christakopoulos P, Karnaouri A. (2018) Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal lytic polysaccharide monooxygenases Biotechnol Biofuels 11(1): 296.

14. Matsakas L, Karnaouri A, Rova U, Christakopoulos P. (2018) Formation of nanoparticles from organosolv isolated lignin from birch biomass Molecules 23:7.

13. Zouraris D, Dimarogona M, Karnaouri A, Topakas E, Karantonis A. (2018) Direct electron transfer of LPMOs and determination of their formal potentials by large amplitude Fourier transform alternating current cyclic voltammetry Bioelectrochemistry 124: 149-155.

12. Karnaouri A, Topakas E, Matsakas L., Rova U, Christakopoulos P. (2018) Fine-tuned enzymatic hydrolysis of organosolv pretreated forest materials for the efficient production of cellobiose. Front. Chem. 6:128.

11. Chalima A, Oliver L, de Castro L.F, Karnaouri A, Dietrich T, Topakas E. (2017) Utilization of volatile fatty acids from microalgae for the production of high added value compounds Fermentation 3(4), 54.

10. Karnaouri A, Muraleedharan MN, Dimarogona M, Topakas E, Rova U, Sandgren M, Christakopoulos P. (2017) Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates, Biotechnol Biofuels 10:126.

9. Karnaouri A, Lange H, Crestini C, Rova U, Christakopoulos P. (2016) Chemoenzymatic fractionation and characterization of pretreated birch outer bark, ACS Sustainable Chem. Eng. 4 (10):5289–5302.

8. Nitsos C, Stocklosa R, Karnaouri A, Voros D, Lange H, Hodge D, Crestini C, Rova U, Christakopoulos P. (2016) Isolation and characterization of organosolv and alkaline lignins from hardwood and softwood biomass ACS Sustainable Chem. Eng. 4 (10):5181–5193.

7. Karnaouri A, Rova U, Christakopoulos P. (2016) Effect of different pretreatment methods on birch outer bark: New biorefinery routes. Molecules 21(4):427.

6. Karnaouri A, Matsakas L, Topakas E, Rova U, Christakopoulos P. (2016) Development of thermophilic tailor-made enzyme mixtures for the bioconversion of agricultural and forest residues, Front Microbiol. 7:177.

5. Karnaouri A, Topakas E, Antonopoulou I, Christakopoulos P. (2014) Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila, Front. Microbiol. 5:281.

4. Karnaouri A, Topakas E, Christakopoulos P. (2014) Cloning, expression, and characterization of a thermostable GH7 endoglucanase from Myceliophthora thermophila capable of high-consistency enzymatic liquefaction. Appl. Microbiol. Biotechnol. 98(1):231-42.

3. Κarnaouri A, Topakas E, Paschos T, Taouki I, Christakopoulos P. (2013) Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila. PeerJ 26;1: e46.

2. Nikolopoulos GK, Masgala A, Tsiara C, Limitsiou OK, Karnaouri AC, Dimou NL, Bagos PG. (2011) Cytokine gene polymorphisms in multiple sclerosis: a meta-analysis of 45 studies including 7379 cases and 8131 controls. Eur J Neurol. 18 (7): 944–951.

1. Bagos PG, Karnaouri AC, Nikolopoulos GK, Hamodrakas SJ. (2007) No evidence for association of CTLA-4 gene polymorphisms with the risk of developing multiple sclerosis: a meta – analysis. Mult. Scler. 13 (2): 156 – 68.

Book Chapters

Karnaouri A, Zerva A, Christakopoulos P, Topakas E. (2020) Screening of recombinant lignocellulolytic enzymes through rapid plate assays. Methods in Molecular Biology, Springer, Series Ed.: Walker, John M., ISSN: 1064-3745.

International Conference Attendances

32. Rova U., Karnaouri A., Matsakas L., Christakopoulos P. Enzyme-mediated production of prebiotic cello-oligosaccharides from forest biomass. Oral presentation, BERSTIC 2020: II International Congress on Biorefineries and Renewable Energies, Santander, Colombia, 2020.

31. Christakopoulos P, Karnaouri A, Matsakas L, Rova U. Valorization of forest biomass cellulosic fraction towards the production of prebiotic oligosaccharides through biocatalysis. Oral presentation, IV-International Conference on ‘Sustainable Energy & Environmental Challenges’ (IV-SEEC), Nagpur, Maharashtra, India, 2019.

30. Karnaouri A, Matsakas L, Krikigianni E, Buhler S, Rova U, Christakopoulos P. Enzyme-mediated production of prebiotic cello-oligosaccharides from forest biomass. Oral presentation, Biospectrum-2019, Thiruvalla, Kerala, India, 2019.

29. Kalogiannis K, Michailof M, Lappas A, Karnaouri A, Chalima A, Topakas E. Mild oxidative organosolv pretreatment of lignocelullosic biomass residues for high added value chemicals and food additives via fermentation processes. Oral presentation, 17th International Conference on Chemistry and the Environment, Thessaloniki, Greece, 2019.

28. Jalvo Sanchez B, Karnaouri A, Matsakas L, Rova U, Christakopoulos P, Mathew A. Isolation and characterization of tunicin nanocrystals from modified tunicate biomass. Oral Presentation, 7th International Conference on Biobased and Biodegradable Polymers, Stockholm, Sweden, 2019.

27. Asimakopoulou G, Karnaouri A, Perraki D, Kalogiannis K, Lappas A, Topakas E. Efficient lactic acid production through conversion of organosolv pretreated lignocellulosic biomass by Lactic Acid Bacteria. Oral presentation, 7th International Conference on Sustainable Solid Waste Management, Crete, Greece, 2019.

26. Paz A, Karnaouri A, Templis C, Papayannakos N, Topakas E. Biorefinery of olive pomace waste: optimization of Organosolv pretreatment process. Poster presentation, 7th International Conference on Sustainable Solid Waste Management, Crete, Greece, 2019.

25. Dedes G, Karnaouri A, Triantafyllidis K, Topakas E. Development of novel chemo-enzymatic processes for the conversion of lignocellulosic biomass towards the production of furans. Poster presentation, 7th International Conference on Sustainable Solid Waste Management, Crete, Greece, 2019.

24. Chalima A, Karnaouri A, Topakas E. Optimization of DHA production from the heterotrophic microalga Crypthecodinium cohnii utilizing a dark fermentation effluent. Oral presentation, 7th International Conference on Sustainable Solid Waste Management, Crete, Greece, 2019.

23. Karnaouri A, Kostopoulos P, Chalima A, Kalogiannis K, Lappas A, Topakas E. Utilization of lignocellulosic biomass for the production of omega-3 fatty acids by the marine microalgae Crypthecodinium cohnii. Oral presentation, 7th International Conference on Sustainable Solid Waste Management, Crete, Greece, 2019.

22. Asimakopoulou G, Karnaouri A, Perraki D, Kalogiannis K, Lappas A, Topakas E. Lactic acid production via high gravity enzymatic hydrolysis and fermentation by lactic acid bacteria, Poster presentation, 8th Congress of the Scientific Society “MikroBioKosmos”, Rio – Patras, Greece, 2019.

21. Zouraris, M. Dimarogona M, Karnaouri A, Topakas E, Karantonis A. Study of H2O2 kinetics of an immobilized Lytic Polysaccharide Monooxygenase with FTacV. Poster presentation, XXV International Symposium on Bioelectrochemistry and Bioenergetics, Limerick Ireland, 2019.

20. Karnaouri Α, Jalvo Sanchez Β, Moritz P, Matsakas L, Rova U, Mathew A, Hoefft O, Sourkouni – Argirusi G, Maus-Friedrichs W, Christakopoulos P. LPMO-assisted preparation of oxidized nanocellulose with high carboxyl content from tunicate biomass. Oral presentation, 41th Symposium on Biotechnology for Fuels and Chemicals, Seattle, 2019.

19. Kalogiannis K, Kalogianni A, Michailof C, Topakas E, Karnaouri A, Lappas A. Single step organosolv oxidative pretreatment of lignocelullosic biomass for valorisation to high added value chemicals and food additives. 68th Canadian Chemical Engineering Conference, Toronto, Canada, 2018.

18. Bossard Ε, Karnaouri Α, Tsafantakis Ν, Aligiannis Ν, Assimopoulou Α, Topakas Ε, Fokialakis Ν. Environmentally-friendly enzyme-assisted extraction of naphtoquinones from Alkanna tinctoria roots. Oral presentation, 30th International Symposium on the Chemistry of Natural Products, Athens, Greece, 2018.

17. Karnaouri A, Muraleedharan MN, Zouraris D, Karantonis A, Topakas E, Sandgren M, Rova U, Christakopoulos P. Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal lytic polysaccharide monooxygenases. Oral presentation, 2nd LPMO Symposium, Marseille, France, 2018.

16. Karnaouri A, Matsakas L, Sanchez BJ, Mathew A, Noren F, Christakopoulos P, Rova U. Organosolv based biorefinery for the valorization of tunicate for food, material and fuel production. Oral Presentation, BIOSD 2018 Conference, Hyderabad, India, 2018.

15. Chalima A, Karnaouri A, Topakas E. Utilization of Crypthecodinium cohnii for the production of PUFA using biowaste derived VFA as main carbon sources in a pH-auxostat, 6th International Conference on Sustainable Solid Waste Management, Naxos, Greece, 2018.

14. Karnaouri A, Matsakas L, Nitsos C, Noren F, Rova U, Christakopoulos P. Exploring abundant marine ascidian bioresources for second generation bioethanol and food-grade prebiotic oligosaccharide production. Poster presentation, 40th Symposium on Biotechnology for Fuels and Chemicals, Florida, 2018.

13. Matsakas L, Karnaouri A, Rova U, Christakopoulos P. Formation of nanoparticles from organosolv isolated lignin from birch biomass. Poster presentation, 40th Symposium on Biotechnology for Fuels and Chemicals, Florida, 2018.

12. Karnaouri A, Topakas Ε, Rova U, Christakopoulos P. Tailoring enzymatic mixtures for the efficient production of cellobiose from organosolv pretreated forest materials, Oral Presentation, FP1306 COST Action 4th Workshop & 5th MC Meeting, Thessaloniki, Greece, 2018.

11. Chalima A, Fafoutis A, Karnaouri A, Topakas E. Utilization of Chlorella zofingiensis for the production of astaxanthin using lignocellulose derived sugars and VFAs as carbon sources, Oral presentation, 2nd Training School, ESSEM COST Action ES1408, European network for algal-bioproducts (EUALGAE), Almeria, Spain, 2017.

10. Karnaouri A, Lange H, Crestini C, Rova U, Christakopoulos P, Chemoenzymatic fractionation and characterization of pretreated birch outer bark, Oral Presentation, FP1306 COST Action WG1/WG3 Meeting, Lisbon, Portugal, 2016.

9. Karnaouri A, Rova U, Christakopoulos P, Designing processes for valorization of forest materials: the case of birch outer bark, Oral Presentation, FP1306 COST Action 2nd Training School, Leipzig, Germany, 2016.

9. Karnaouri A, Lange H, Crestini C, Rova U, Christakopoulos P, Chemoenzymatic fractionation and characterization of pretreated birch outer bark, Oral Presentation, FP1306 COST Action WG1/WG3 Meeting, Lisbon, Portugal, 2016.

8. Karnaouri A, Tyrel L, Rova U, Christakopoulos P, Physicochemical fractionation and enzymatic treatment of birch outer bark towards the evaluation of ‘oleochemicals’ and ‘active’ ingredients. Oral Presentation, 38th Symposium on Biotechnology for Fuels and Chemicals, Baltimore, 2016.

7. Karnaouri A, Rova U, Christakopoulos P, Effect of physicochemical pretreatment on fractionation of lignin/suberin-rich birch outer bark, Oral Presentation, FP1306 COST Action WG1/WG3 Meeting, Litvinov, Czech Republic, 2015.

6. Muraleedharan MN, Karnaouri A, Dimarogona M, Topakas E, Rova U, Christakopoulos P, Development of tailor-made ‘oxidative boosted’ enzyme mixtures for the bioconversion of targeted feed stocks, Poster Presentation, 11th Carbohydrate Bioengineering Meeting (CBM11), Helsinki, Finland, 2015.

5. Karnaouri A, Matsakas L, Muraleedharan MN, Topakas E, Rova U, Christakopoulos P, Optimization of tailor-made enzyme cocktail for deconstruction of agricultural and forest residues. Poster Presentation, 37th Symposium on Biotechnology for Fuels and Chemicals, San Diego, 2015

4. Karnaouri A, Topakas E, Christakopoulos P, A thermostable GH7 endoglucanase from Myceliophthora thermophila capable of high-consistency enzymatic liquefaction, Poster Presentation, 10th Carbohydrate Bioengineering Meeting (CBM10), Prague, Czech Republic, 2013.

3. Karnaouri A, Topakas E, Christakopoulos P, Molecular cloning, expression and characterization of a thermostable GH7 endoglucanase from Myceliophthora thermophila with high-consistency liquefaction properties, Oral Presentation, Bio4energy Research School, Skelleftea, Sweden, 2013.

2. Karnaouri A, Topakas E, Christakopoulos P, Cloning, heterologous expression and characterization of cellulolytic enzymes from the thermophilic fungi Myceliophthora thermophila, Poster Presentation, Plant and Seaweed Polysaccharides Symposium (PSP 2012), Nantes, France, 2012.

1. Karnaouri A, Topakas E, Christakopoulos P, Cloning, heterologous expression and characterization of a family 7 thermophilic endoglucanase from Myceliophthora thermophila (StEG7), Poster Presentation, 9th Carbohydrate Bioengineering Meeting (CBM9), Lisbon, Portugal, 2011.